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Title :
Determination the serum level creatinin

Objective
To Determination the serum level creatinin

Principle :
Glycin ——(liver)– > creatin —– > creatin ph —– > creatin —- > kidney

+ arginin

Procedure
1 ml of working solution in test tubes and 1 ml in standard tube and 1 ml in Blank tube
– 100 m of serum in test tebes
– 100 m of standard in st tubes
– 100 m of D.Win B tube
. incubation for 20 min at 25 C
. read O.D against Blank at 546 nm .
* normal leve =0.7 – 1.1 mg dl

Result :
Concentration ot test = A(t) x 2 mg dl
A(s)
= 0.096 x 2 = 1.57 mg dl
0.122
Discussion and conclsion:
Serum og level creatinin high
. abnormal
. renal defect

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